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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
09/11/2017 |
Actualizado : |
12/12/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Soil and rhizosphere bacterial diversity in maize agro-ecosystem. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 35-51. |
ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
DOI : |
10.5539/sar.v6n3p |
Idioma : |
Inglés |
Notas : |
Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. |
Contenido : |
ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial ... Presentar Todo |
Palabras claves : |
DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668
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Marc : |
LEADER 02541naa a2200289 a 4500 001 1057747 005 2018-12-12 008 2017 bl uuuu u00u1 u #d 022 $a1927-050X (Print) // 1927-0518 (Online) 024 7 $a10.5539/sar.v6n3p$2DOI 100 1 $aFEDERICI, M. 245 $aSoil and rhizosphere bacterial diversity in maize agro-ecosystem.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. 520 $aABSTRACT. Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices. Copyright © Canadian Center of Science and Education 653 $aDGGE 653 $aMETAGENOMICS 653 $aPYROSEQUENCING 653 $aRHIZOSPHERE 653 $aSOIL BACTERIA 653 $aTRANSGENIC MAIZE 700 1 $aBAJSA, N. 700 1 $aLAGURARA, P. 700 1 $aREVALE, S. 700 1 $aMARCONDES, J.A. 700 1 $aDALLA RIZZA, M. 773 $tSustainable Agriculture Research, 2017$gv.6 (3): 35-51.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
21/02/2014 |
Actualizado : |
27/05/2021 |
Tipo de producción científica : |
Documentos |
Autor : |
BONILLA, O.; ZORRILLA DE SAN MARTÍN, G.; DEAMBROSI, E.; ROVIRA, P.J.; BERMÚDEZ, R. |
Afiliación : |
OSCAR RUBEN BONILLA PIRIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GONZALO ROBERTO ZORRILLA DE SAN MARTÍN PEREYRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE GERMAN DEAMBROSI CHURRUT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAÚL ENRIQUE BERMÚDEZ COQUARD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Unidad de Producción Arroz - Ganadería. |
Fecha de publicación : |
2003 |
Fuente / Imprenta : |
In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Treinta y Tres. Producción de carne vacuna y ovina de calidad. Seminario de actualización técnica. Treinta y Tres (Uruguay): INIA, 2003. |
Páginas : |
p. 45-49 |
Serie : |
(INIA Serie Actividades de Difusión ; 317) |
Idioma : |
Español |
Thesagro : |
ARROZ; GANADERIA; PRODUCCION DE CARNE; URUGUAY. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/335/1/14445020908145851.pdf;ad317#page=50
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Marc : |
LEADER 00778naa a2200229 a 4500 001 1007573 005 2021-05-27 008 2003 bl uuuu u00u1 u #d 100 1 $aBONILLA, O. 245 $aUnidad de Producción Arroz - Ganadería. 260 $c2003 300 $ap. 45-49 490 $a(INIA Serie Actividades de Difusión ; 317) 650 $aARROZ 650 $aGANADERIA 650 $aPRODUCCION DE CARNE 650 $aURUGUAY 700 1 $aZORRILLA DE SAN MARTÍN, G. 700 1 $aDEAMBROSI, E. 700 1 $aROVIRA, P.J. 700 1 $aBERMÚDEZ, R. 773 $tIn: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Treinta y Tres. Producción de carne vacuna y ovina de calidad. Seminario de actualización técnica. Treinta y Tres (Uruguay): INIA, 2003.
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